| Selektro A/S | Central Denmark Region |
| Semaphor | Capital Region of Denmark |
| Semark | Capital Region of Denmark |
| SEMO | Capital Region of Denmark |
| Sentia Danmark A/S | Capital Region of Denmark |
| Sentispec ApS | |
| SEO Denmark | |
| SEO-1 | |
| Sepior ApS | |
| Serious Games Interactive | Capital Region of Denmark |
| ServicePartner Danmark A/S | Capital Region of Denmark |
| Shareit | Region of Southern Denmark |
| Shield Intelligence | |
| Shopbox | |
| Shouter | |
| Sif Gruppen A/S | Capital Region of Denmark |
| Silkeborg Data | |
| sima.dk A/S | Region of Southern Denmark |
| SimaTech A/S | |
| SimCorp | Capital Region of Denmark |
| Simple Solution P/S | |
| SimpleSite | Capital Region of Denmark |
| Simply.com | Capital Region of Denmark |
| Simsoft | |
| SITECH Danmark A/S | |
| Siteimprove A/S | |
| Skanderby A/S | Central Denmark Region |
| Skåne Sjælland Linux Users Group | Region Zealand |
| Skive College | Central Denmark Region |
| Skjern Bank | Central Denmark Region |
| Skjoldby & Co ApS | Region Zealand |
| Skysolution ApS | |
| Sl-software | Capital Region of Denmark |
| Sleeknote ApS | |
| SmartResponse | |
| SmartShare Systems A/S | Capital Region of Denmark |
| SmartTID | Region of Southern Denmark |
| SmartWeb | |
| SMT Data A/S | |
| Snipr Biome | |
| Soft Design | Capital Region of Denmark |
| SoftBASE A/S | |
| SoftControl | |
| Softpay ApS | |
| SoftwareONE Denmark Aps | |
| Softwork | Region Zealand |
| Solitwork | |
| Solution Group ApS | |
| Solution IT ApS | |
| SolutionWeb | Capital Region of Denmark |