| VIAWEB.es | Madrid |
| VICOMTECH | |
| Vida Ventures | |
| VidaApp | Madrid |
| Video Sound Solutions | Madrid |
| Vidjet | Barcelona |
| Viewed | Madrid |
| Viewtinet | Madrid |
| Vigartel | Valencia |
| Vigo Marketing | Pontevedra |
| Vilamedi Servicios Informáticos | Barcelona |
| Villae | Madrid |
| Vincle | Barcelona |
| Vincusys, S.L. | A Coruña |
| Viral Studios | Badajoz |
| Viralgen | |
| Viralgen Vector Core | Gipuzkoa |
| Vircell, S.L. | |
| Virtual Software, S.L. | Madrid |
| VirtualDomus.com | Barcelona |
| Virtualitze | Barcelona |
| Virtualtec.es | Barcelona |
| Virtuarch studio S.L.P. | |
| Virtuimagen | Biscay |
| Virtuosity Spain, S.L. | Barcelona |
| Virtusway | |
| Visibli | |
| Visiotech | Madrid |
| Visual MicroSystems | Pontevedra |
| Visual Publinet, S.L. | A Coruña |
| Visual Trans | Pontevedra |
| Visualfy by Fusió d'Arts | Valencia |
| VisualNACert | |
| VisualNEO | Madrid |
| Visualtis, S.L. | Murcia |
| Visualweb | Tarragona |
| Vitaance | |
| Vitamina K Venture Capital S.C.R., S.A. | Madrid |
| Vitelsa | Madrid |
| Viterbit | Madrid |
| Vitro, S.A. | Sevilla |
| Viupik Visuals, S.L. | |
| VIVEBiotech | Gipuzkoa |
| Vivia Biotech, S.L. | Madrid |
| Vivotecnia | Gipuzkoa |
| Vixiees | |
| Vizologi | Alicante |
| VjInformatica Servicios Integrales de Informatica | Alicante |
| VLC Photonics, S.L. | Valencia |
| VLEX Networks, S.L.U. | |