| Tufin | Tel Aviv District |
| Tuki | Central District |
| Tunefork | Jerusalem District |
| Tweak | Tel Aviv District |
| TweetBookz | Tel Aviv District |
| Tyro | |
| TytoCare Ltd | Central District |
| TZUR Ltd | Central District |
| UAE-IL tech zone | Tel Aviv District |
| UBTECH | Central District |
| UDI User Interface Design Ltd | Tel Aviv District |
| UI | Tel Aviv District |
| UK Israel Tech Hub | Tel Aviv District |
| Umoove | Tel Aviv District |
| UNI - Sophisticated Electronic Assembling Ltd | Tel Aviv District |
| Unicloud | Central District |
| Unicon | Tel Aviv District |
| Unifabrix | |
| Unilink Ltd | Tel Aviv District |
| UNIPaaS Payments Technologies | Tel Aviv District |
| Unipharm Ltd | Tel Aviv District |
| Unit Group | Central District |
| United States-Israel Binational Science Foundation | |
| Unitronics | |
| Universal McCANN Digital | Tel Aviv District |
| University of Haifa | Haifa District |
| University of Haifa - Bioinformatics | |
| University of Haifa - Caesarea Rothschild Institute for Interdisciplinary Applications of Computer Science | Haifa District |
| University of Haifa - Department of Computer Science | Haifa District |
| University of Haifa - Department of Mathematics | Haifa District |
| University of Haifa - Israeli Society for Bioinformatics and Computational Biology | |
| UP | Tel Aviv District |
| UP-IT | |
| Updated Company Ltd | Central District |
| Upright Technologies Ltd | Tel Aviv District |
| Urban Aeronautics | |
| Urban Digital | Central District |
| Urban Place | Tel Aviv District |
| US-Israel Binational Agricultural Research and Development Fund | |
| USBid | Tel Aviv District |
| USBShop.co.il | Tel Aviv District |
| Usearch | Tel Aviv District |
| UVID Drontech | |
| Uvision | |
| V-CHECK | Central District |
| V-Check Ltd | Central District |
| V-HOLA | Central District |
| V-Wave Ltd | Haifa District |
| Valence Security | Tel Aviv District |
| Valens | Central District |