| BCST S.r.l. | Monza and Brianza |
| BdSound S.r.l. | |
| Be Safe Group S.r.l. | Rome |
| Be. Shaping the Future S.p.A. | |
| beanTech S.r.l. | Friuli-Venezia Giulia |
| Bebit S.r.l. | |
| beCAD S.r.l. | |
| Becom S.r.l. | Brescia |
| BeDigital S.r.l. | |
| Beeapp | Pisa |
| Beentouch S.r.l. | Catania |
| BeeSoft S.r.l. | Ascoli Piceno |
| Beintoo S.p.A. | Milan |
| Bellani S.a.s. | La Spezia |
| Bemycompany S.r.l. | |
| Bending Spoons S.p.A. | |
| Bentel Security S.r.l. | Teramo |
| BeonD S.r.l. | |
| Beretta S.r.l. | Monza and Brianza |
| BestNetwork | Rome |
| Beta Elettronica S.r.l. | Bergamo |
| BetaGlue Technologies S.p.A. | |
| BeTeck Soc. Coop. | South Sardinia |
| Bett Sistemi S.r.l. | Reggio Emilia |
| BEV | Milan |
| Beyond The Box | Milan |
| BFF Banking Group | Milan |
| BFT S.p.A. | Vicenza |
| Bi Elle S.r.l. | Turin |
| BI@Work S.r.l. | Friuli-Venezia Giulia |
| Bieffedue Elettronica S.r.l. | Parma |
| Biella Linux User Group | Biella |
| BIELLE S.a.s. | Rome |
| BigProfiles | Rome |
| Bio4Dreams S.p.A. | |
| Biocode Bioinformatics | Rome |
| Biofarma S.r.l. | |
| Bioindustry Park Silvano Fumero S.p.A. | Turin |
| BIOMAS S.r.l. | Bologna |
| Biometric S.r.l. | Turin |
| Biometrika S.r.l. | Forlì-Cesena |
| Bios Elettronica S.r.l. | Rome |
| BiovelocITA S.r.l. | |
| Bisognin | Vicenza |
| Bit2win S.r.l. | |
| Bit4id S.r.l. | |
| BitBang | Bologna |
| BitBoss S.r.l. | |
| Bitnet S.r.l. | Rome |
| BitSong S.r.l. | |