| Expert Systems | Queensland |
| Expert360 | New South Wales |
| Explanimate | Queensland |
| Expose Media | Queensland |
| Exposure By Design | |
| Express Virtual Meetings | Victoria |
| Extensity | Western Australia |
| Ey3 | Queensland |
| Eye Candy Media | Queensland |
| Eye Jam Multimedia | New South Wales |
| Eyo Technologies Pty Ltd | New South Wales |
| EzeScan | Queensland |
| Ezidox | New South Wales |
| ezimerchant | New South Wales |
| EzTools Software | New South Wales |
| Ezy2c | New South Wales |
| ezyLAN | New South Wales |
| EzyMsg.com | Queensland |
| FAB9 | Victoria |
| Facet Consulting | Queensland |
| Facilitate | Western Australia |
| FACTS Online Pty Ltd | Victoria |
| FacturaDirecta | Victoria |
| Faethm | New South Wales |
| Fair Trade Power Pty Ltd | |
| Fairport | Western Australia |
| FaktorTel | Queensland |
| Fanix Software | Queensland |
| Fantastic One Pty Ltd | New South Wales |
| Farmbot | New South Wales |
| Fast IT Solutions | Queensland |
| Fast Track Computer Solutions | Western Australia |
| Fastmail | Victoria |
| FastTrack Pty Ltd | Victoria |
| Fat Digital | New South Wales |
| Fat Rhino Design | Victoria |
| Fenwick Software | Victoria |
| Ferocia | |
| Ferronova | South Australia |
| Fexle Services Pty Ltd | New South Wales |
| Fifth Domain Pty Ltd | Australian Capital Territory |
| Fileshake Digital | Western Australia |
| Financial Mappers | Queensland |
| Finch | Victoria |
| Find-Me Technologies Pty Ltd | Queensland |
| FindMap | New South Wales |
| Fingerprint for Success | New South Wales |
| Finite Recruitment | New South Wales |
| Finley Sliced | Western Australia |
| FinSS Global | Victoria |