| Advanced Navigation | New South Wales |
| Advanced User Systems Pty Ltd | New South Wales |
| AdvanCell Isotopes Pty Ltd | New South Wales |
| Advansys Pty Ltd | New South Wales |
| Advantage Computer Systems | Western Australia |
| Advantage iT | New South Wales |
| Advantech Software | Queensland |
| ADVIS | Victoria |
| Advisible | New South Wales |
| AdWise Digital | Victoria |
| Aegis | Victoria |
| Aerion Technologies Pty Ltd | Victoria |
| Aerometrex | South Australia |
| AffClicks | |
| Affirm Software | Victoria |
| AFK | New South Wales |
| Africa-11.com | Tasmania |
| AGDATA Australia | Queensland |
| Agency Iceberg Pty Ltd | Victoria |
| Agent Oriented Software Pty Ltd | Victoria |
| Agile Communications | South Australia |
| Agile Computing Pty Ltd | Western Australia |
| Agile Digital Engineering Pty Ltd | Australian Capital Territory |
| Agile SEO | Queensland |
| Agileware Pty Ltd | Australian Capital Territory |
| Agilex Biolabs Pty Ltd | South Australia |
| Agilient | New South Wales |
| AGIS For Windows | Tasmania |
| AGIX | South Australia |
| Agora Commerce | Victoria |
| AGP Investment Management Ltd | |
| AgriWebb | New South Wales |
| Aguawebs | New South Wales |
| AH Technology (AUST) Pty Ltd | Victoria |
| AI Brisbane | Queensland |
| Ai Tel Pty Ltd | South Australia |
| AI-Media | |
| Aiculus Pty Ltd | Victoria |
| AiiMS | New South Wales |
| AIM Software Pty Ltd | Victoria |
| AINS | Victoria |
| AirBorn Electronics | New South Wales |
| AirTree Ventures Pty Ltd | New South Wales |
| Airtronic Circuits Pty Ltd | Victoria |
| AirTrunk Operating Pty Ltd | New South Wales |
| Airwallex | Victoria |
| AJAXWorkspace | Western Australia |
| Akaal Pharma Pty Ltd | Victoria |
| Akips | |
| Akra Web | New South Wales |