| Kronos Australia Pty Ltd | New South Wales |
| KSM Solutions | New South Wales |
| Ktec Solutions Pty Ltd | New South Wales |
| La Trobe University | Victoria |
| La Trobe University - Department of Computer Science & Computer Engineering | Victoria |
| La Trobe University - Department of Mathematics and Statistics, Bendigo | Victoria |
| Langdale Consultants | New South Wales |
| Language Technology and Data Analysis Laboratory | Queensland |
| Lanrex | New South Wales |
| Lantana Systems Pty Ltd | Victoria |
| Laptop Plus | South Australia |
| Laptop.com.au Pty Ltd | Victoria |
| Lastek Pty Ltd | South Australia |
| Lateral Aspect | Western Australia |
| Lateral Pharma Pty Ltd | Victoria |
| Latina Girls Code | |
| Latitude Financial Services | |
| Launceston Computer Group, Inc. | Tasmania |
| Launch Pad - Western Sydney University | New South Wales |
| Launchpad | |
| LaunchVic | Victoria |
| Lava Software Pty Ltd | South Australia |
| LawMaster | Queensland |
| Lawsoft Pty Ltd | South Australia |
| Lawtech (Australia) Pty Ltd | Queensland |
| LBT Innovations | South Australia |
| Leader Cloud | South Australia |
| LeadFactory | New South Wales |
| Leading Technology | Victoria |
| LeadKing Digital | Queensland |
| LEAP Legal Software | New South Wales |
| Leapfrog Market | |
| Leda Electronics Pty Ltd | Western Australia |
| LEDsales | Tasmania |
| Legend | New South Wales |
| Lennox Computer Software Design | Queensland |
| Let's Build a Website Pty Ltd | New South Wales |
| Lexer | Victoria |
| Lexian Solutions | New South Wales |
| Liberating Solution | |
| Libero | Queensland |
| Light Source Digital Pty Ltd | |
| Lightbulb Digital | South Australia |
| Lighterra | Queensland |
| Lighthouse Business Innovation Centre | Australian Capital Territory |
| Lightplay | New South Wales |
| Limepay | New South Wales |
| LinearG | New South Wales |
| Link Digital | Australian Capital Territory |
| LinkageWiz | South Australia |