| ESG Electronic Service Ges.m.b.H. | Lower Austria |
| Esselbach Internet Solutions | Vienna |
| eSYS Informationssysteme GmbH | |
| Eternal Storms Software | Vienna |
| Eticon | Carinthia |
| ETRON Softwareentwicklungs- und Vertriebs GmbH | Lower Austria |
| Ettico Labs | |
| EUCODIS Bioscience GmbH | |
| Eumedics Medizintechnik und Marketing GmbH | Vienna |
| Eurofunk Kappacher GmbH | Salzburg |
| Eurolyser Diagnostica GmbH | |
| Euromsoft | Styria |
| euronorm | Vienna |
| Europe mpo International Consulting GmbH | Styria |
| European Alternatives | |
| Eveliqure Biotechnologies GmbH | |
| Eveliqure Biotechnologies GmbH | |
| EVER Pharma | Upper Austria |
| Evercyte GmbH | |
| Eversberg | Salzburg |
| Everyone Codes gGmbH | |
| Excellence Research | |
| Exertis Pro AV Austria GmbH | Salzburg |
| EXLIBRIS Software & Multimedia GmbH | Upper Austria |
| expressflow | Vienna |
| Eyeson GmbH | |
| Eyeson GmbH | |
| Fa. Robert Schön | Vienna |
| Fabasoft AG | Upper Austria |
| FACC AG | |
| Fachhochschule des BFI Wien | Vienna |
| Fachhochschule Kärnten | Carinthia |
| Fachhochschule Technikum Wien | Vienna |
| Fachhochschule Vorarlberg GmbH | Vorarlberg |
| Fachhochschule Wiener Neustadt GmbH | Lower Austria |
| Fact AI GmbH | |
| factor4 Internetagentur | Salzburg |
| Factory Hub Vienna | |
| Fait Internet Software GmbH | Vienna |
| Fast Lane Institute for Knowledge Transfer GmbH | Vienna |
| FAW GmbH | Upper Austria |
| Feedback Fish | |
| Feiba Engineering & Plants GmbH | Upper Austria |
| Felbermayr Holding GmbH | |
| FeldTech GmbH | Styria |
| Felgo | |
| Feller GmbH | Lower Austria |
| Fellinger & Co GmbH | Upper Austria |
| Female Founders | |
| Feragen | Salzburg |