| BlackIP | South Holland |
| Blackorange | |
| Blanco Services B.V. | |
| Blauwe Zone | Friesland |
| Blender | North Holland |
| Blits B.V. | |
| Bloqhouse | North Holland |
| Blue dot VATBox Ltd | |
| Blue Dragon | North Brabant |
| Blue Innovation Center | Limburg |
| BlueBased B.V. | Gelderland |
| Bluebird Day | North Brabant |
| BlueHealth Innovation Center VZW | |
| Bluelink B.V. | North Holland |
| BluePolar | North Holland |
| Blueprint Cyber Security | |
| BluMedialab | North Holland |
| BM Webshops B.V. | |
| Bohemian B.V. | South Holland |
| Bol ICT | Groningen |
| Boldly-XR | North Brabant |
| Bonami Games & Computers Museum | Overijssel |
| Bondex IO | |
| Bons IT Solutions | Limburg |
| Boo2 | North Holland |
| Booking.com B.V. | North Holland |
| BOOM Digital | |
| Booreiland | North Holland |
| Boostrz | Overijssel |
| Borca Online Computer Service | South Holland |
| BORG-OSC | South Holland |
| Bosor B.V. | North Brabant |
| Boss B.V. | North Holland |
| BOSS Company B.V. | North Holland |
| Bosweb.nl | South Holland |
| BouwInfosys | North Brabant |
| BoVli Solutions | |
| Boxis | Gelderland |
| Boyz in the Cloud | |
| BP-Web | |
| Bradford Engineering B.V. | North Brabant |
| BrainDonors | |
| Braingineers International B.V. | |
| Brainport Eindhoven | North Brabant |
| BrainSys | |
| Brainwave Systems | North Holland |
| Brama Systems B.V. | |
| Bratpack | North Holland |
| Braventure | North Brabant |
| Bravo | Limburg |