| AllSolutions | Utrecht |
| Allyourmedia | Gelderland |
| ALM computers | Utrecht |
| Almanapp B.V. | |
| Alopex Elektronica | South Holland |
| Alot Hosting | Gelderland |
| Alpha Robotica | South Holland |
| Alpha.One B.V. | |
| Alphabeats | North Brabant |
| Alphacomm B.V. | |
| Alphamega | North Brabant |
| AlpInvest Partners B.V. | |
| ALSO International B.V. | Gelderland |
| AlthA B.V. | Gelderland |
| AltusHost B.V. | |
| Always be learning | |
| am+d brand management B.V. | South Holland |
| AM-Pharma B.V. | |
| Amarna Therapeutics | South Holland |
| Amazec Photonics | |
| Amber Implants B.V. | |
| Amberscript Global B.V. | |
| Ambrero Software B.V. | North Holland |
| Amcom B.V. | North Holland |
| Amdax | North Holland |
| America-Direct B.V. | South Holland |
| Amitron B.V. | South Holland |
| AMMP Technologies B.V. | |
| Amphebia | South Holland |
| Amphera | |
| Ampleon | |
| Amrif | |
| Amsterdam Capital House | |
| Amsterdam Health & Technology Center | |
| Amsterdam Internet Exchange | North Holland |
| Amsterdam Smart City | North Holland |
| Amsterdam Trade Bank | North Holland |
| Amsterdam University College | North Holland |
| Amsterdam University of Applied Sciences | North Holland |
| Amsterdam Venture Partners | North Holland |
| Amsterdam Venture Studios | North Holland |
| Amsterdams Instituut voor ArbeidsStudies | |
| Amsterdamse Hogeschool voor de Kunsten | |
| Amyyon Software | Groningen |
| Anadolubank Nederland N.V. | North Holland |
| AnalogFolk | |
| Anavo Therapeutics B.V. | |
| Anchormen | North Holland |
| Anders-IT | |
| Andes | North Brabant |