| Urbandine B.V. | |
| Urbee | Utrecht |
| URLfilterDB B.V. | Gelderland |
| Usabilityweb.nl | North Holland |
| Usabilla B.V. | North Holland |
| Uselab | North Holland |
| Usenet4u B.V. | Limburg |
| Userguest | North Holland |
| USoft B.V. | North Holland |
| Utilis | |
| Utomik B.V. | North Brabant |
| Utrecht University - Department of Information and Computing Sciences | Utrecht |
| UtrechtInc | |
| V.S.M. Automatisering B.V. | North Brabant |
| V4-Automatisering | Gelderland |
| VAA Data Works | North Brabant |
| Vabi | South Holland |
| Vactik | Utrecht |
| Valadis B.V. | Overijssel |
| Valid | North Brabant |
| Valk Solutions | Utrecht |
| Vallei-ICT | Gelderland |
| VallueBlue | Utrecht |
| ValueGiG | North Brabant |
| Valyuu B.V. | South Holland |
| Van Beek Elektrotechniek B.V. | Utrecht |
| Van den Broek Systemen B.V. | North Brabant |
| Van Dulst Automatisering | North Brabant |
| Van Eldijk | Utrecht |
| van Gelder Systems | |
| Van Gils Computersystemen | North Brabant |
| Van Room Automatisering | Flevoland |
| Van Tuinen Webdesign | Drenthe |
| van Zon Internet | North Brabant |
| Vanderlande Industries B.V. | |
| VanDerLee | North Holland |
| Vanguard Entertainment Group | North Holland |
| VanMoof B.V. | North Holland |
| Varenkamp & Jansen Automatisering | Zeeland |
| VarmX B.V. | |
| VascoGames B.V. | |
| VBcentral | Gelderland |
| VBIT | |
| VBVB | North Holland |
| VC4A | |
| vConsultant | |
| VDX | Overijssel |
| Vectis Invest B.V. | |
| VectorY Therapeutics | North Holland |
| Veenman B.V. | Utrecht |