| Evestra Onkologia | |
| Evestra Onkologia Sp. z o.o. | |
| Evide | Masovian |
| EVR-Systems | Lower Silesian |
| eWIPO | Masovian |
| EWITRYNA Sp. J. | Łódź |
| Ewosoft | Lesser Poland |
| EWPOL Automatyka Przemysłowa | Lower Silesian |
| Exago Media | |
| Exanet Sp. z o.o. Sp. k. | Greater Poland |
| eXant Software Polska Sp. z o.o. | Greater Poland |
| EXATEL, S.A. | Masovian |
| EXCOM Breza Hanna | Kuyavian-Pomeranian |
| Exeon | |
| EXEQ Sp. z o.o. Sp. k. | |
| Exeria | |
| Existek | |
| Exnit Sp. z o.o. | Lesser Poland |
| Expanse Agency | Łódź |
| Expansio | Greater Poland |
| Experior Venture Fund | Masovian |
| Experssive-One | Greater Poland |
| ExploRNA Therapeutics Sp. z o.o. | |
| Expose Sp. z o.o. | Masovian |
| Expro | Silesian |
| Extel Networking Sp. z o. o. | Lesser Poland |
| F&F Filipowski Sp. J. | Łódź |
| F.H.U. Progreson | Silesian |
| F.H.U. Telvicom | Greater Poland |
| F1 IT Consultancy | Pomeranian |
| Fabrity Sp. z o.o. | |
| Fabryka Stron | Opole |
| FabrykaStron | West Pomeranian |
| Fair Place Finance | |
| Faith-Net S.C. | Lesser Poland |
| Falcom Sp. z o.o. | Lublin |
| Falcon Games | |
| Falcon V Systems | |
| Faldruk S.C. | Masovian |
| Fandla Faktoring Sp. z o.o. | |
| Fandroid | Lower Silesian |
| Fast IT Sp. z o.o. Sp. k. | Łódź |
| FastLogic | Łódź |
| Faxiko Sp. z o.o. | Warmian-Masurian |
| FCA Sp. z o.o. | Lesser Poland |
| Fdgstudio.net | Silesian |
| Felcad | Silesian |
| FELG Software Sp. z o.o. | Lesser Poland |
| Fenige S.A. | |
| Ferguson | Greater Poland |