| Pohoda-Servis spol. s r.o. | South Bohemian |
| POINT.X Mobility, s.r.o. | Prague |
| Pojišťovna VZP, a.s. | |
| Polovodiče, a.s. | South Moravian |
| Polytechna Consulting, a.s. | |
| Position, s.r.o. | Prague |
| PostSharp | Prague |
| PowerHUB | |
| PowerHUB z.ú. | |
| PPF banka, a.s. | Prague |
| Prague IoT Centre & SmartCity inkubátor | Prague |
| Prague Startup Centre | Prague |
| Premisoft, s.r.o. | |
| Presto Ventures | |
| Primehammer, s.r.o. | |
| PrivacyRoot, s.r.o. | Prague |
| Profi Credit Czech, a.s. | |
| Profinet, s.r.o. | |
| Profinit EU, s.r.o. | Prague |
| Profitux.cz | Liberec |
| ProfiWH | Olomouc |
| Proidea.digital | Ústí nad Labem |
| Prointernet, s.r.o. | South Bohemian |
| ProjectSoft HK, a.s. | |
| PROKOP software, s.r.o. | Prague |
| PromethistAI, a.s. | |
| Promotic | Moravian-Silesian |
| Prospeks-IT.cz | |
| Protean, s.r.o. | |
| Protronix, s.r.o. | Pardubice |
| Prusa Research, a.s. | |
| PS Media s.r.o. | Moravian-Silesian |
| PUX, s.r.o. | South Moravian |
| PyCon CZ | Moravian-Silesian |
| PZK Brno, a.s. | |
| Q-net CZ | South Moravian |
| Q2 Interactive, s.r.o. | South Moravian |
| QCM | South Moravian |
| Qore Technologies, s.r.o. | Prague |
| Quant Retial, s.r.o. | Central Bohemian |
| Quantasoft | Prague |
| Quantcom | |
| Quantum Phi, s.r.o. | |
| Qubit Conference | Prague |
| Queen's Software | Olomouc |
| Query Digital, s.r.o. | Prague |
| Quinta-Analytica, s.r.o. | |
| Racom, s.r.o. | Prague |
| Radek Chalupa | Prague |
| Raiffeisenbank | Prague |