| 1click Webdesigns | Cornwall |
| 1ComputerCare | Kent |
| 1CSI Ltd | |
| 1Design 4Life | Kent |
| 1Ecommerce | Flintshire |
| 1LG Digital | Buckinghamshire |
| 1minus1 Ltd | Surrey |
| 1PCS Ltd | Northamptonshire |
| 1Spatial | Cambridgeshire |
| 1st Aid Repairs | Kent |
| 1st autorun express | Wiltshire |
| 1st Byte IT Solutions | North Yorkshire |
| 1st Callcom Ltd | Derbyshire |
| 1st Class Media | Edinburgh |
| 1st Clock | |
| 1st Direct | |
| 1st DNS Ltd | Greater London |
| 1st for Web Hosting | Cardiff |
| 1st Plus Web Design | |
| 1st Rate Comms Ltd | East Sussex |
| 1st Step Recruitment | |
| 1st Technologies Ltd | |
| 1st Voice Solutions Ltd | Essex |
| 1stWebDesigns | Leicestershire |
| 1UPDesign | West Midlands |
| 2-Minute-Website Ltd | Greater London |
| 20-20 Web Design | South Yorkshire |
| 200OK Solutions | Greater London |
| 2020viz Web Design | Buckinghamshire |
| 205 Media Ltd | |
| 20VC Fund | |
| 21 Six Ltd | |
| 210 Digital | |
| 2112 Direct Marketing | |
| 21Digital Ltd | Lancashire |
| 21six Ltd | Hampshire |
| 21st Century New Media Ltd | Hertfordshire |
| 22i | Hampshire |
| 23snaps Ltd | |
| 23Squared Ltd | Hampshire |
| 24 Seven Communications Ltd | West Yorkshire |
| 247 Commerce Ltd | Greater London |
| 24X Ltd | West Sussex |
| 26 DX | |
| 2able | Dorset |
| 2Big2Send Ltd | Oxfordshire |
| 2CL Communications Ltd | Hampshire |
| 2Dmedia Ltd | Essex |
| 2e-volve (UK) Ltd | Stirling |
| 2iS Consulting Ltd | |