| ElitSat, s.r.o. | Bratislava |
| Elnec, s.r.o. | Prešov |
| ELPRO, s.r.o. | Košice |
| Elumi Studio | |
| Empire Systems, s.r.o. | |
| EMtest, a.s. | |
| EMware a.s. | Žilina |
| Ene-Tep, a.s. | |
| Energia - Jarás, s.r.o. | |
| Energo-Aqua, a.s. | |
| Ensentia, s.r.o. | |
| Entry Net | Bratislava |
| Epix | Trnava |
| ErasData-Pro | |
| ErlaServers, s.r.o. | |
| eROBOT, j.s.a. | |
| ESET | Bratislava |
| ESET, spol. s r.o. | Bratislava |
| ESMO Žilina, a.s. | Žilina |
| eSolutions, s.r.o. | Košice |
| Espio, s.r.o. | |
| eStranky.sk | Bratislava |
| Etarget SE | Bratislava |
| EUDomains.sk | Bratislava |
| Euro Media Košice, s.r.o. | |
| Euronet | Trenčín |
| Európsky inštitút ďalšieho vzdelávania | |
| EuroReklama.sk | Bratislava |
| EuroServer.sk | Banská Bystrica |
| Evelon, s.r.o. | Bratislava |
| exe.sk | Bratislava |
| Exel, s.r.o. | Banská Bystrica |
| EXO hosting | Prešov |
| Exo Technologies, spol. s r.o. | Bratislava |
| EXOT, s.r.o. | Bratislava |
| FatChilli.com | Bratislava |
| Fevra, spol. s r.o. | |
| FileJet | |
| Filip M & W, spol. s r.o. | Bratislava |
| Fine, s.r.o. | Žilina |
| FLEX-IS, spol. s r.o. | Bratislava |
| Flowy | |
| Focus-computer, spol. s .r.o. | |
| Foliovision, s.r.o. | Bratislava |
| Fond inovácií a technológií, a.s. | |
| Fonet, spol. s r.o. | Trenčín |
| Foresta Sk, a.s. | |
| Forma, s.r.o. | |
| Forsam, spol. s r.o. | |
| FortKnox Personal Firewall | Trenčín |