| Ekspress Hotline | Harju |
| Ektaco AS | Harju |
| Elering AS | |
| Eliko | |
| Elisa Eesti AS | Harju |
| Elkdata OÜ | Harju |
| ELKIS Grupp OÜ | Harju |
| ELKO Grupa AS | Harju |
| Ellie | |
| ElmoSoft, LLC | |
| Elogic Commerce | |
| Elvior | Harju |
| Emercoin | |
| Empresa En Estonia OÜ | Harju |
| Epilocal OÜ | |
| ERPLY Books | |
| ESA BIC Estonia | Harju |
| EssentialPIM | Harju |
| EST Analytics OÜ | |
| EstBAN | Harju |
| Ester Digital OÜ | |
| ESTO AS | |
| Estonian Business and Innovation Agency | |
| Estonian Business School | |
| Estonian Entrepreneurship University of Applied Sciences | |
| Estrola OÜ | Harju |
| Eternet | |
| eu-LISA | Harju |
| Eurolink OÜ | Harju |
| European Innovation Academy | |
| Eurora | |
| Evedo.co | |
| EVNE Developers | |
| EvoBlox OÜ | |
| Exillery Ltd | Harju |
| Extranet Oü | |
| EyeVi Technologies | |
| EZ Exchange OÜ | |
| Fairown | |
| Falconers | Harju |
| Fastboss | |
| FASTVPS Eesti OÜ | |
| Faunder | Harju |
| Feanor OÜ | Harju |
| Feedreader | Harju |
| FibroTx | |
| FIIZY Group | |
| Filosoft | Harju |
| Filter Forge OÜ | |
| Finantsinspektsioon | |