| Virgilio Media | New Jersey |
| Virgin Galactic | California |
| Virgin Hyperloop | California |
| Virgin Pulse | Rhode Island |
| Virginia BioTechnology Research Park | Virginia |
| Virginia Broadband, LLC | Virginia |
| Virginia Commercial Space Flight Authority | |
| Virginia Commonwealth University Center for High Performance Computing | Virginia |
| Virginia Commonwealth University Computer Science Department | Virginia |
| Virginia Commonwealth University Department of Mathematics and Applied Mathematics | Virginia |
| Virginia DeBolt | Texas |
| Virginia Innovation Partnership Corporation | Virginia |
| Virginia Institute of Marine Science | Virginia |
| Virginia Israel Advisory Board | |
| Virginia Macintosh Users Group | Virginia |
| Virginia Military Institute | Virginia |
| Virginia Polytechnic Institute and State University | Virginia |
| Virginia Polytechnic Institute and State University Department of Computer Science | Virginia |
| Virginia Polytechnic Institute and State University Department of Mathematics | Virginia |
| Virginia Semiconductor, Inc. | Virginia |
| Virginia State University | Virginia |
| Virginia Systems | Virginia |
| Virginia Tech | Virginia |
| Virginia Tech Corporate Research Center | Virginia |
| Virginia Tech Department of Electrical and Computer Engineering | Virginia |
| Virginia Tech Department of Mathematics | Virginia |
| Virginia Tech Fralin Biomedical Research Institute | Virginia |
| Virginia Technology Services, LLC | Virginia |
| Virginia Union University | Virginia |
| Virginia University of Lynchburg | Virginia |
| Virginia University of Science and Technology | Virginia |
| Virginia Wesleyan University | Virginia |
| Virgo Web Design | |
| Virial International, LLC | Maryland |
| Viridian Therapeutics, Inc. | Massachusetts |
| Viridio | California |
| Viridis Terra International, Inc. | |
| Virigina MicroComputer Services | Virginia |
| Virima, Inc. | Georgia |
| Viriom, Inc. | California |
| Virion Therapeutics, LLC | |
| VirnetX Holding Corporation | Nevada |
| ViroGen | Rhode Island |
| Vironexis Biotherapeutics | |
| Virongy | Virginia |
| Virool | California |
| ViroStat, Inc. | Maine |
| Virovek | Texas |
| VirPack | Virginia |
| Virpax Pharmaceuticals | |