| National Defense Magazine | Virginia |
| National Discount Merchant Services | |
| National Energy Research Scientific Computing Center | California |
| National Environmental Satellite, Data, and Information Service | Maryland |
| National Exchange Carrier Association, Inc. | New Jersey |
| National Geospatial-Intelligence Agency | Virginia |
| National Grid Partners | |
| National Human Genome Research Institute | |
| National Information Solutions Cooperative, Inc. | Missouri |
| National Initiative for Cybersecurity Careers and Studies | District of Columbia |
| National Institute for Computational Sciences | Tennessee |
| National Institute of Aerospace | |
| National Institute of Biomedical Imaging and Bioengineering | Maryland |
| National Institute of Standards and Technology | Maryland |
| National Institute of Standards and Technology - Computer Security Resource Center | Maryland |
| National Institute of Standards and Technology - Math, Statistics and Computational Sciences | Maryland |
| National Institute of Statistical Sciences | |
| National Institutes of Health | |
| National Instruments Corporation | Texas |
| National Intelligence University | District of Columbia |
| National Louis University | Illinois |
| National Optical Instruments, Inc. | Texas |
| National Optronics | Texas |
| National Park College | Arkansas |
| National Payment Processing | California |
| National Photonics Initiative | |
| National Positions.com | California |
| National Quantum Institute | District of Columbia |
| National Recalls | California |
| National Reconnaissance Office | |
| National Renewable Energy Laboratory | Colorado |
| National Research Corporation | |
| National Resilience, Inc. | California |
| National Rural Telecommunications Cooperative | Virginia |
| National Science Foundation | Virginia |
| National Security Agency | Maryland |
| National Security Technology Accelerator | |
| National Software Escrow, Inc. | Ohio |
| National Switchgear | Texas |
| National Technical Information Service | Virginia |
| National Technologies | Illinois |
| National Telecommunications and Information Administration | |
| National Telecommunications Cooperative Association | Virginia |
| National Telephone & Technology, Inc. | Maine |
| National Venture Capital Association | District of Columbia |
| National Video Works | California |
| National Web Designers | |
| NationalSalesBooster | California |
| NationBuilder | California |
| Nations Wholesale Company, Inc. | North Carolina |