| Barrenjoey | New South Wales |
| Barton Brands | Queensland |
| BaseHost | Victoria |
| Basepair Genomics | Victoria |
| Baseplan Software Group | New South Wales |
| Bateleur Consulting | New South Wales |
| Bathurst Software Solutions | New South Wales |
| BAXE | Queensland |
| Bay Technologies Pty Ltd | Queensland |
| Baymatob Operations Pty Ltd | New South Wales |
| Bayview Technologies Pty Ltd | Victoria |
| BCS Technology International Pty Ltd | New South Wales |
| BDM Digital Marketing | Queensland |
| Be by Animoca Brands Pty Ltd | |
| Beagle Internet Pty Ltd | New South Wales |
| Beforepay Group Ltd | New South Wales |
| Beige | Victoria |
| Bellarine Mac User Group | Victoria |
| Bellsoft Technologies | New South Wales |
| Benchmark Global Pty Ltd | New South Wales |
| Bendigo and Adelaide Bank Ltd | Victoria |
| Bendigo Telco Ltd | Victoria |
| Bento | |
| BES IT Systems | Queensland |
| Best Deal 4 U Computers & I.T. Pty Ltd | Queensland |
| Best Practice Software | Queensland |
| Best Telecom Group | Queensland |
| Better Software Pty Ltd | South Australia |
| Beyond Bank Australia Ltd | South Australia |
| Beyond Essential Systems Pty Ltd | Victoria |
| Beyondedge | Victoria |
| BGL Corporate Solutions Pty Ltd | Victoria |
| BHL Software | New South Wales |
| Big Head Digital | Western Australia |
| BigDataCloud Pty Ltd | South Australia |
| BigPulse Voting Pty Ltd | New South Wales |
| Bigstone | New South Wales |
| BigTurnHR | New South Wales |
| Biko Technologies Pty Ltd | New South Wales |
| Bill Hero Holdings Pty Ltd | |
| Binary Ninja | |
| Binary Studio Pty Ltd | New South Wales |
| BinaryLane | |
| biNu Pty Ltd | New South Wales |
| BioCina | South Australia |
| BioDiem Ltd | Victoria |
| BiomeBank | South Australia |
| Biometix Pty Ltd | New South Wales |
| Biometrics Institute Ltd | New South Wales |
| Bionomics Ltd | South Australia |