| Biotron Ltd | New South Wales |
| BirdBrain | Western Australia |
| Bissano Software | Queensland |
| Bit Loud Digital | Victoria |
| Bitcoin.com.au | New South Wales |
| BitScope | New South Wales |
| Bitten by Design | New South Wales |
| Bitunivex | New South Wales |
| Biza Pty Ltd | Queensland |
| BizCatalyst | Victoria |
| Bizpep | Queensland |
| Biztactix | Victoria |
| Bizz Wizz Software | Western Australia |
| BJ Communications | Victoria |
| BJ2DESIGN | New South Wales |
| Bjango Pty Ltd | Victoria |
| Black Sheep Capital | Queensland |
| Blackbird Ventures | New South Wales |
| Blackbook Artificial Intelligence | Queensland |
| Blacklight Productions Pty Ltd | New South Wales |
| Blake Digital | Western Australia |
| Blaq | New South Wales |
| Blend AI | |
| Blinq Technologies Pty Ltd | Victoria |
| Blis Web Agency | South Australia |
| Bliss Media | Victoria |
| Block Earner | New South Wales |
| Blockchain Australia | Victoria |
| Blockchain Technologies | New South Wales |
| Bloocow | Western Australia |
| Bloom Digital | Western Australia |
| BloomIT Consulting Pty Ltd | New South Wales |
| Bloomtools | Queensland |
| Blue Cherry | Western Australia |
| Blue Crystal Solutions | South Australia |
| Blue Line Software Pty Ltd | New South Wales |
| Blue Poppy Ventures | South Australia |
| Blue Star Direct | New South Wales |
| Blue Zoo | Western Australia |
| Blueblood Solutions | Australian Capital Territory |
| BlueChilli | New South Wales |
| Bluegem | Western Australia |
| BlueShield Technologies Pty Ltd | Victoria |
| Blueshyft Pty Ltd | New South Wales |
| BlueSoap | |
| Bluestarfish Web Design | New South Wales |
| Bluewire Media Pty Ltd | Queensland |
| BMC | Western Australia |
| BMYG Asset Management Pty Ltd | Victoria |
| BNS Group | Australian Capital Territory |