| Utixo Ltd | Greater Manchester |
| UTN Training | West Yorkshire |
| Utopia P2P Ecosystem | |
| UUconstruct | Hertfordshire |
| UWC Atlantic | |
| UWS Creative Computing Technologies | |
| UX Connections | |
| UXB London | |
| UXLI | |
| V Formation Ltd | |
| V-Count | |
| V-Nova | |
| V-Nova | |
| V1 Ltd | Cheshire |
| V1 Technologies Ltd | West Lothian |
| V7 | Greater London |
| V8Media Ltd | Herefordshire |
| VA Software Solutions | |
| VA Technology Ltd | |
| Vaarst | |
| Vaccitech plc | |
| VacV Biotheraputics (UK) Ltd | |
| Vadavision Ltd | |
| Vaioni | |
| VaioRefurbs Ltd | |
| Val-U-Computers | Norfolk |
| Valarian Technologies Ltd | |
| Vale Systems | |
| Valen Digital | |
| Valentino's Displays Ltd | |
| Valia | Greater London |
| Valiant Design Ltd | |
| Valiant Design Ltd | |
| ValiRx plc | |
| Vallibel Finance plc | |
| Value Power Systems Ltd | Shropshire |
| Value Web Designs | |
| ValueShop | Greater Manchester |
| Valve Space Ltd | |
| Vamosa | Glasgow |
| Van der Velde (Service and Support) Ltd | |
| VanderWijk Consultancy | Oxfordshire |
| Vanilla Circus Ltd | |
| Vanilla Electronics | Norfolk |
| VanillaPlus | Kent |
| Vannquish Creative | |
| Vantage Biosciences Ltd | |
| Vanti Ltd | |
| Vapour Media Ltd | |
| VAR Capital | |