| Ellipsiz Ltd | Singapore |
| Elms Industrial Pte Ltd | Singapore |
| Elves Lab Pte Ltd | Singapore |
| EmailJS Pte Ltd | |
| Ematic Solutions Pte Ltd | Singapore |
| Emedia Solution Pte Ltd | |
| Emerald Clinical Trials | |
| Emeritus | |
| EmiNET Pte Ltd | Singapore |
| Employ Borderless | |
| EMURGO | |
| Emurgo Pte Ltd | Singapore |
| Enable Computing | Singapore |
| EnableX | Singapore |
| Encore eServices | Singapore |
| Energeia Labs Pte Ltd | Singapore |
| ENGINE Analytics | |
| Engine Biosciences Pte Ltd | Singapore |
| Enjin | Singapore |
| Enjinstarter | |
| Enleofen Bio Pte Ltd | Singapore |
| Ennovatez Group | Singapore |
| eNova Technologies Pte Ltd | Singapore |
| Enspire Capital Pte Ltd | Singapore |
| Enterprise Products Integration Pte Ltd | Singapore |
| Enterprise Singapore | Singapore |
| Entropica Labs | Singapore |
| Entrust Network | Singapore |
| Eoan Systems | Singapore |
| Epecom Graphics | Singapore |
| EPLAN Software & Service | Singapore |
| Epoint Systems Pte LTd | Singapore |
| ePrime Solutions Pte Ltd | Singapore |
| EPS Ventures | Singapore |
| Epson Singapore Pte Ltd | Singapore |
| EQ Brand Pte Ltd | |
| Equatorial Space Systems Pte Ltd | Singapore |
| Equinet Academy | Singapore |
| Equinox Showcase | Singapore |
| ERC Institute | |
| Eruditus | Singapore |
| Esco Aster Pte Ltd | |
| Esco VacciXcell | Singapore |
| ESE Industries (S) Pte Ltd | Singapore |
| Esri Singapore | Singapore |
| ETact Solutions Pte Ltd | Singapore |
| EthAum Venture Partners | Singapore |
| Ethlas | |
| eTrail Web Space | |
| Eunoia Pte Ltd | Singapore |