| QBSW, a.s. | Bratislava |
| R-DAS | Bratislava |
| R-DAS | |
| RadioLAN | |
| Rainside, s.r.o. | Bratislava |
| Rare Crew | Bratislava |
| RASCO, s.r.o. | Bratislava |
| Re Public Group, s.r.o. | |
| RealSoft, s.r.o. | Trenčín |
| Redis, s.r.o. | |
| Renner | Prešov |
| Research Center for Quantum Information | Bratislava |
| Resitech | |
| Responsee | Bratislava |
| RESULT reklamná agentúra, s.r.o. | |
| REVIS, s r.o. | Trenčín |
| RF elements, s.r.o. | |
| Riešenia, spol. s r.o. | Bratislava |
| Rinners.sk, s.r.o. | |
| RMC, s.r.o. | |
| Robotec | |
| Robotic SK, s.r.o. | |
| Robotika.sk | Bratislava |
| RodoComp | Košice |
| ru3ch interactive, s.r.o. | |
| Run Group, s.r.o. | Žilina |
| RVmagnetics, a.s. | |
| S&T Slovakia, s.r.o. | Bratislava |
| S.A.K., spol. s r.o. | Banská Bystrica |
| S.I.G.N., s.r.o. | Bratislava |
| SAE - Automation, s.r.o. | Trenčín |
| SafeStuffs | |
| SAMO Automation, s.r.o. | |
| SanaClis, s.r.o. | |
| Sanding, s.r.o. | |
| Saneca Pharmaceuticals, a.s. | |
| SANET | Bratislava |
| Sapie | Bratislava |
| SATRO, s.r.o. | Bratislava |
| SATTVA, s.r.o. | Banská Bystrica |
| SB systém | |
| Scheidt & Bachmann Slovensko, s.r.o. | |
| SCR design | |
| SCR interactive | Bratislava |
| SCR technologies | Banská Bystrica |
| Screenshot Machine | |
| Screenshotmachine | |
| ScrumDesk | Prešov |
| Seges, s.r.o. | Bratislava |
| Segron Automation, s.r.o. | |