| Urban Systems | |
| Urban Turtle | Quebec |
| Urban Web Designs | British Columbia |
| Urbana Corporation | |
| Urbanimmersive, Inc. | |
| UrbanLogiq | British Columbia |
| Uresta | |
| Uroboro | |
| URSA Marketing | |
| Ursula Comeau | Ontario |
| Ursula2U | Quebec |
| Urubu Studio | Quebec |
| UsabilityReviews.com | Ontario |
| USB Canada | Quebec |
| Used Computer Shop | Quebec |
| Used Monitor Shop | Quebec |
| UsefulParts.com | Ontario |
| User Friendly Systems, Inc. | Ontario |
| UserBase Technologies, Inc. | Ontario |
| Userful Corporation | Alberta |
| Userproof Software Services Ltd | Ontario |
| Usewalter | Quebec |
| UTEX Scientific Instruments, Inc. | Ontario |
| Utherverse Digital, Inc. | British Columbia |
| UtilCall | Ontario |
| Utilimax, Inc. | Quebec |
| UTM Canada | |
| Utopia Image | Quebec |
| Utopia VR | |
| UUUDesign.com | Ontario |
| UV Designs | |
| UW Blueprint | |
| UX Guys, Inc. | Alberta |
| Uxbridge Computer Solutions | Ontario |
| Uzado, Inc. | Ontario |
| Uzinakod | Quebec |
| V-Mobile Software | Ontario |
| V3 Digital, Inc. | Quebec |
| V3 Mediaworks | British Columbia |
| V4IDEAS, Inc. | |
| V51 Consulting, Inc. | Ontario |
| Vagrant Web & Creative | New Brunswick |
| VAKCER | Ontario |
| Valcom Consulting Group, Inc. | Ontario |
| Valcourt 2030 | |
| Valence | |
| Valence Discovery | |
| Valence Labs | Quebec |
| Valentino Marketing, Inc. | Ontario |
| Valeo Pharma | Quebec |