| Overit | |
| Overjet | Massachusetts |
| Overland AI | Washington |
| Overland Therapeutics (US), Inc. | |
| Overlap Interactive, LLC | |
| Overlay | Texas |
| Overlode, Inc. | Colorado |
| Overlook Systems Technologies, Inc. | Virginia |
| Overman & Associates | Ohio |
| Overnight PC Repair | California |
| OverNite Software, Inc. | Texas |
| OverOps, Inc. | California |
| Oversecured | |
| Oversight | Georgia |
| Overstreet Associates | |
| OverT Bio, Inc. | |
| Overtime Sports, Inc. | |
| Overture | |
| Overture Life | New York |
| Overture Orthopaedics | |
| OverTurn Technologies | California |
| Overview.ai | California |
| Overwatch Data | |
| Overwatch Data Services | Michigan |
| Overwatch Imaging | |
| Oviedo Medical Research | |
| Ovitas | Massachusetts |
| Oviva Therapeutics, Inc. | |
| OVNI Capital | |
| OVO Fund | California |
| Ovo Studios, LLC | Ohio |
| OWASP Foundation, Inc. | Massachusetts |
| OWDT | Texas |
| Owen & Walker Software Solutions | Texas |
| Owens Consulting | California |
| Owkin, Inc. | New York |
| Owl Biomedical, Inc. | California |
| Owl Cameras, Inc. | California |
| Owl Capital Group | California |
| OWL Computing, Inc. | Michigan |
| Owl Cyber Defense | Connecticut |
| Owl Labs | Massachusetts |
| Owl Peak Labs | |
| Owl Peak Technologies | |
| OWL Testing Software | Pennsylvania |
| Owl Therapeutics | |
| Owl Ventures | California |
| Owlchemy Labs | Texas |
| Owler | |
| Owler, Inc. | California |