| SpaceAG | Florida |
| SpaceAlpha Insights Corporation | |
| SpaceApp | |
| Spaceblue, LLC | Ohio |
| SpaceCom | |
| SpaceCom 2022 | Virginia |
| SpaceCraft | Colorado |
| Spacedesign Corporation | Texas |
| SpaceDev | |
| SpaceDev | |
| Spacee, Inc. | Texas |
| SpaceFab.US | |
| SpaceFund Venture Capital | |
| SpaceGroundAmalgam, LLC | |
| SpaceIntel | Ohio |
| Spaceium | |
| Spacek Labs, Inc. | California |
| SpaceKnow, Inc. | |
| Spacelabs Healthcare | Washington |
| Spacelift | California |
| SpaceNav | |
| SpaceNews | Virginia |
| Spaceport | Massachusetts |
| Spaceport America | |
| Spacesmack | |
| Spacestar | Minnesota |
| Spaceti | |
| Spacetime, LLC | |
| SpaceWare, Inc. | Texas |
| SpaceWorks Enterprise, Inc. | Georgia |
| SpaceX | California |
| Spacial AI, Inc. | |
| Spacio, Inc. | |
| Spade Data, Inc. | New York |
| Spainhower, LLC | Colorado |
| Spalding Software, Inc. | Georgia |
| Spalding University | Kentucky |
| Spam-Filter.com | Massachusetts |
| Spamex | New York |
| Span Enterprises, LLC | Arkansas |
| Span Global Services | |
| Span.IO, Inc. | California |
| Spanawave Corporation | California |
| Spandrel Interactive, Inc. | |
| Spanios | Texas |
| Spanitz Consulting | Illinois |
| Spanning Cloud Apps, LLC | Texas |
| Spanning Cloud Apps, LLC | |
| SPAR Information Systems | |
| Spara, Inc. | |