| Speeds | Tel Aviv District |
| Spera Security | Tel Aviv District |
| Spetrotec Ltd | |
| Spikerz | |
| Spincare | |
| Spinner Design Studio | Central District |
| Spiral Solutions Interactive Marketing | |
| Spiralic | |
| SpliSense | Jerusalem District |
| SPO Precision Optics | |
| SpotNet | |
| Spring Games | Tel Aviv District |
| Spring Ventures | Tel Aviv District |
| SPS Smart Printing Solutions Ltd | Central District |
| SQLink Group | Tel Aviv District |
| SRV Internet Services | Tel Aviv District |
| StageOne Ventures | Tel Aviv District |
| StageOne Ventures | |
| StairwaySoft Ltd | |
| Stands Ltd | Tel Aviv District |
| Star Farm Ventures | |
| Star Ventures | Tel Aviv District |
| StarkWare Industries | Central District |
| Start Israel | Tel Aviv District |
| Start-Up Nation Central | Tel Aviv District |
| Start-Up Nation Finder | Tel Aviv District |
| Startup Camel | Tel Aviv District |
| Startup Stash | Tel Aviv District |
| StartupHub.ai | Tel Aviv District |
| State of Israel Ministry of Communications | |
| State Of Mind Ventures | Tel Aviv District |
| SteelKiwi, Inc. | Tel Aviv District |
| Step Of Mind Ltd | Tel Aviv District |
| Sternum | Tel Aviv District |
| Stero Biotechs Ltd | |
| STG Digital Marketing | |
| Stier Ltd | |
| StoreDot | |
| StoreYa Feed Ltd | |
| Stratasys Ltd | Central District |
| Stratigo Creative Consultants | Central District |
| Stratoscale | Tel Aviv District |
| Stream.Security | Tel Aviv District |
| Strigo | Tel Aviv District |
| StuccoMedia | Tel Aviv District |
| StuffThatWorks | |
| SuperCom | Tel Aviv District |
| Supercreator.ai | |
| SuperSmart | Tel Aviv District |
| Superwise | Tel Aviv District |