| Retriever | New South Wales |
| Revelation Software Concepts Pty Ltd | Victoria |
| Revium | Victoria |
| Rewardle Pty Ltd | Victoria |
| Rezdy | New South Wales |
| Rhipe Ltd | New South Wales |
| Rhombus Solutions | Victoria |
| Rhythm Biosciences | Victoria |
| Rhythm Biosciences Ltd | Victoria |
| Ridge Films Corporate Pty Ltd | |
| Rift Software | Western Australia |
| Right Click Capital Management Pty Ltd | |
| Right Lane | Victoria |
| Ripefruit Media | Victoria |
| Riseweb Pty Ltd | Victoria |
| Rising Software | Victoria |
| Risk Wizard Pty Ltd | Victoria |
| River City Labs | Queensland |
| RiverPitch | Queensland |
| RMIT University | Victoria |
| RMIT University - Computer Science | Victoria |
| RMS Cloud | Victoria |
| Roadnight Web Dynamics | Victoria |
| Roar Local Pty Ltd | New South Wales |
| Robert Walters Australia | New South Wales |
| Robological Pty Ltd | |
| Robotron Group | Victoria |
| Rock Agency | Victoria |
| Rockby | Victoria |
| Rocket Remit | Australian Capital Territory |
| Rocket Seeder Ltd | Victoria |
| RocketBoots | New South Wales |
| RODIN Business Solutions | New South Wales |
| ROI Growth Agency | Victoria |
| ROI Growth Agency | |
| Rojone Pty Ltd | New South Wales |
| Roland DG Australia Pty Ltd | New South Wales |
| ROM Technologies | New South Wales |
| Ron Savage | Australian Capital Territory |
| Roster Portal | Queensland |
| Rotapix Interactive Media | New South Wales |
| Roundhouse | Queensland |
| RST Cloud Pty Ltd | New South Wales |
| Rubber Tree Software | New South Wales |
| Rubicon Red | Queensland |
| RuleQuest Research Pty Ltd | New South Wales |
| Runway HQ | Victoria |
| Rural Systems | South Australia |
| RxPx | New South Wales |
| S1T2 | |