| Shark Digital | |
| Shernet Integrated Services | New South Wales |
| Sherpa | New South Wales |
| Shift Financial Pty Ltd | New South Wales |
| Shifted Pixels | New South Wales |
| Shine Solutions Group | Victoria |
| Shippit | New South Wales |
| Shiv Technolabs | New South Wales |
| Shivam Technologies | Victoria |
| Shivam Web Design | Victoria |
| Shoalhaven Internet | New South Wales |
| Shorthand Pty Ltd | Queensland |
| Shotstack Pty Ltd | New South Wales |
| Shout Agency | |
| Sibagraphics | Queensland |
| Sibedge | |
| SideProjectors | |
| Silicon Cloud International | South Australia |
| Silicon Quantum Computing | New South Wales |
| Silver Communications | Victoria |
| Simient Pty Ltd | Queensland |
| Simple KYC Pty Ltd | New South Wales |
| Simple Net Australia Pty Ltd | Victoria |
| Simple Pages | Queensland |
| Simple Retail | New South Wales |
| SimpleDiagrams | Tasmania |
| Simply Savvy Web | Western Australia |
| Simply Wall Street Pty Ltd | New South Wales |
| simPRO Group Pty Ltd | Queensland |
| Singtel Optus Pty Ltd | New South Wales |
| SIS Industrial Cyber Security | Victoria |
| Sitback Solutions | New South Wales |
| Sitelabs | Victoria |
| SiteMinder | New South Wales |
| SitePoint Pty Ltd | Victoria |
| Sites n Stores | Victoria |
| SiteSuite Website Design | New South Wales |
| SiteZero | Queensland |
| SK8Tech | New South Wales |
| Skalata Ventures Pty Ltd | |
| Skandus Pty Ltd | New South Wales |
| Sked Social | Victoria |
| SkedGo | New South Wales |
| Sketch That | Victoria |
| Skin2Neuron Pty Ltd | New South Wales |
| Skip Capital | New South Wales |
| Skript, Inc. | New South Wales |
| Sky and Space Company Ltd | Western Australia |
| Skyfii Ltd | New South Wales |
| SkyNet Satellite Communications Pty Ltd | Queensland |