| Spark Green | Victoria |
| Spark Interact | New South Wales |
| Sparklabs Cultiv8 Pty Ltd | New South Wales |
| Sparro Digital Marketing | New South Wales |
| Sparx Systems Pty Ltd | Victoria |
| Spatial Industries Business Association | Australian Capital Territory |
| Spatial Intelligence | New South Wales |
| Spatial Vision | Victoria |
| Spearhead Software | Western Australia |
| Special K Software | Victoria |
| SpectraQEST | South Australia |
| Speedwell | Queensland |
| SpeeDx Pty Ltd | New South Wales |
| Sphere Pty Ltd | New South Wales |
| Spidersoft | Victoria |
| Spike Innovation | Queensland |
| Spinfluence | New South Wales |
| Spinks Security Pty Ltd | Victoria |
| SpinTel | New South Wales |
| Spirit Telecom Ltd | Victoria |
| Splash | |
| Splashbox | |
| SponsoredLinX | |
| Spoon | Western Australia |
| SpoonFeedMe Pty Ltd | |
| Spot Co-Working | South Australia |
| SpreadsheetGuys.com | New South Wales |
| Sprout Online | Victoria |
| SproutX | Victoria |
| Sqonk Pty Ltd | Victoria |
| Square Peg Capital Pty Ltd | New South Wales |
| Square18 | Western Australia |
| SquareAlpha | Western Australia |
| SQUIDLYdigital | Queensland |
| Squiz | New South Wales |
| SRA Information Technology | Northern Territory |
| SRA Solutions | New South Wales |
| SSW | New South Wales |
| St. George Bank | |
| St. George Web Design | New South Wales |
| Stack Digital | |
| STACK.aero | |
| Stairways Software Pty Ltd | Queensland |
| Staley Automation | Victoria |
| Stamina Software | New South Wales |
| Stanski Group | Victoria |
| Star 3 Media | Western Australia |
| Star Business Solutions | Victoria |
| Star Config | New South Wales |
| Star-tek Technology Pty Ltd | Victoria |