| Starfish Ventures Pty Ltd | Victoria |
| Start Digital | Western Australia |
| StartInno Ventures Pty Ltd | New South Wales |
| Startmate | Victoria |
| Startup Adelaide | South Australia |
| Startup Catalyst Pty Ltd | Queensland |
| Startup Circle | New South Wales |
| Startup Creative | New South Wales |
| Startup Daily | New South Wales |
| Startup Melbourne | Victoria |
| Startup News | Western Australia |
| Startup Victoria | Victoria |
| Startup WA | Western Australia |
| StartupAUS Ltd | New South Wales |
| Startupbootcamp Australia | Victoria |
| Stat Health Systems | Queensland |
| State Government of Victoria Innovation Network | Victoria |
| statistiXL | Western Australia |
| Statronics Power Supplies | New South Wales |
| Steadfast | New South Wales |
| Steadfast Solutions | Victoria |
| Steep Digital | Western Australia |
| Step Ahead Software Pty Ltd | New South Wales |
| Step Change | New South Wales |
| Step Two Designs Pty Ltd | New South Wales |
| Sterland | New South Wales |
| StewArtMedia | Victoria |
| STI Engineering Pty Ltd | Western Australia |
| Stickman | New South Wales |
| Stickyweb Pty Ltd | Victoria |
| Stillwell Select Recruitment | South Australia |
| Stimulus | New South Wales |
| Stingrae | New South Wales |
| StitchCraft | New South Wales |
| Stockspot Pty Ltd | New South Wales |
| Stone & Chalk | New South Wales |
| Stone Digital Pty Ltd | New South Wales |
| Storm Front Technology Pty Ltd | Northern Territory |
| Storm FX Distributions Pty Ltd | New South Wales |
| Straight Path Solutions | Victoria |
| Straightsell | South Australia |
| Stralia Web | New South Wales |
| Strand7 | New South Wales |
| Strategic Consulting Partnerships | |
| Strategic Directions | Queensland |
| Strategic Group | New South Wales |
| Streamtime Software Ltd | New South Wales |
| Strike Media | South Australia |
| Stripy Sock | Australian Capital Territory |
| StrongPoint Web Design Pty Ltd | Queensland |