| Studio 313 | New South Wales |
| Studio 72 | Victoria |
| StudioHawk | Victoria |
| Style Atlas | |
| Sublime HQ Pty Ltd | New South Wales |
| Sublime IP Pty Ltd | New South Wales |
| Sublime Text | |
| Sublime Text | |
| Success Systems Pty Ltd | Queensland |
| Sumico Net | Queensland |
| Summit Internet | Victoria |
| Summit Software Pty Ltd | Victoria |
| Summit Web Perth | |
| Sundata Pty Ltd | Queensland |
| Sunrise Technologies | New South Wales |
| Super A | |
| Super Domains Australia | Victoria |
| SuperConcepts | New South Wales |
| Superior IT | Western Australia |
| Supple | |
| SupplyLink International | Queensland |
| Supportsoft Technologies | New South Wales |
| Supreme Supports Ltd | New South Wales |
| Surety IT | Queensland |
| Surfpacific | Queensland |
| Surpass Computer | Queensland |
| Sushi Digital | |
| SwarmFarm Robotics | Queensland |
| Swift Computers Pty Ltd | New South Wales |
| Swift Digital | New South Wales |
| SwiftTech | Queensland |
| Swinburn University of Technology | Victoria |
| Swinburne University of Technology - Information Technology | Victoria |
| Swish Connect Pty Ltd | Victoria |
| Swish Online | Western Australia |
| Swoop Digital | Victoria |
| Swyvl | South Australia |
| SXiQ | Victoria |
| Sybiz Software | South Australia |
| SYD Ventures | New South Wales |
| Sydney Angels | New South Wales |
| Sydney Antennas | New South Wales |
| Sydney Branding Services | New South Wales |
| Sydney Linux User Group | New South Wales |
| Sydney PC User Group | New South Wales |
| Sydney Search Results Pty Ltd | New South Wales |
| Sydney Startup Hub | New South Wales |
| Sydney Technology Solutions Pty Ltd | New South Wales |
| Symbio Holdings Ltd | New South Wales |
| Symbion Pty Ltd | |