| e-Quest | North Brabant |
| E-sites | North Brabant |
| e-Storage | Utrecht |
| e-UUR | Overijssel |
| E-Works B.V. | South Holland |
| E.B.P. Point of Sale B.V. | North Brabant |
| E.C.C. Stack | |
| EA BEST Websolutions | Limburg |
| Eagle Vision Systems B.V. | North Holland |
| Ease2pay B.V. | |
| Eastern Enterprise | Overijssel |
| Easy Template B.V. | Utrecht |
| Easy2Rent | Utrecht |
| EasyAds B.V. | Utrecht |
| EasyComputing/Mensys B.V. | North Holland |
| EasyData | Gelderland |
| Easyflex | North Brabant |
| Easygenerator B.V. | South Holland |
| Easyhosting B.V. | North Holland |
| Easypos | North Brabant |
| easyusenet | North Holland |
| EATRIS ERIC | |
| EBAP | North Holland |
| EBK | Flevoland |
| ebs Interactive Communications | Flevoland |
| Ebusco | North Brabant |
| Eclarion | Gelderland |
| EclectiC International Consulting | North Brabant |
| Ecochain Technologies B.V. | |
| Ecomation | North Brabant |
| eConcepts | Gelderland |
| eConnect International B.V. | |
| ecOrganizer | North Holland |
| Ecotone | South Holland |
| ECP | South Holland |
| Edenspiekermann | North Holland |
| EDIAS Software International B.V. | South Holland |
| Edition1 | South Holland |
| eDocs | Overijssel |
| edpnet | |
| Eduapp Benelux B.V. | |
| Edubas | Groningen |
| Educagen | North Holland |
| Educom / Detacom | Limburg |
| Eeko Print B.V. | Overijssel |
| Eemland Software & Internet | Utrecht |
| eenSTUDIO | |
| Effect Photonics | North Brabant |
| eformity | South Holland |
| Efty B.V. | |