| Egeniq | Utrecht |
| eHour | North Holland |
| Eigen & Wijze Internet Marketing | Friesland |
| Eigen Domein | Gelderland |
| Eight | Gelderland |
| Eijdems Internet | |
| Eindhoven University of Technology - Department of Mathematics and Computer Science | |
| EIT Health Investor Network | |
| El Niño | Overijssel |
| El-contronic B.V. | Utrecht |
| Elaborate | South Holland |
| Elasticsearch B.V. | North Holland |
| ElCheapo B.V. | |
| Eldra B.V. | Limburg |
| Elect B.V. | Overijssel |
| ElectricIQ B.V. | |
| Elements | Flevoland |
| Elincom Electronics B.V. | South Holland |
| Elitac Wearables | Utrecht |
| Eljes Infrastructurele Projecten B.V. | Flevoland |
| Elka Pieterman Holland B.V. | North Brabant |
| EllageElectronics B.V. | Overijssel |
| Ellogon | |
| ELPROMA Electronics | Utrecht |
| Eltan B.V. | North Brabant |
| Elwave | South Holland |
| EMATTERS | North Holland |
| Emazing Webdesign | Utrecht |
| Embrace | Groningen |
| Emergo Systems B.V. | Gelderland |
| Eminent | Gelderland |
| Emitter Studios B.V. | |
| Emmia B.V. | North Brabant |
| Emons Group B.V. | |
| Emproof B.V. | |
| EMRO Automatisering | Friesland |
| Emultech | |
| Encis ICT | North Holland |
| Endeit Capital | North Holland |
| ENDROiT | North Holland |
| Enformation | North Holland |
| EnGenius Networks Europe B.V. | |
| Engine Software B.V. | Gelderland |
| Enginia B.V. | Utrecht |
| Enigmatry | South Holland |
| Enjoy and Deploy | South Holland |
| Enline Internet Solutions | Limburg |
| Enpicom B.V. | |
| Enreach | Friesland |
| Enrise | Utrecht |