| EvenHosten | |
| eVentures Africa Fund B.V. | |
| EventX | South Holland |
| Evertalent | |
| Everynet | South Holland |
| Everything4Office Software B.V. | South Holland |
| Evi Software B.V. | North Brabant |
| EVO Venture Partners | North Holland |
| Evologics | North Brabant |
| EW Webservices Amersfoort | |
| Exa-Omicron | Flevoland |
| Exact | South Holland |
| Except Integrated Sustainability B.V. | |
| Exedo | South Holland |
| ExeVir Bio B.V. | |
| EXIN | Utrecht |
| Exor | North Holland |
| ExoRobotics | |
| Expansion | South Holland |
| Experience Data | |
| Experis | North Holland |
| Expert Solar Systems | |
| Experthosting.nl | |
| Expice B.V. | North Holland |
| ExSpect | North Brabant |
| Extravar | South Holland |
| eXtremelyWEB | Gelderland |
| Exvo.com B.V. | South Holland |
| Eye Security | |
| Eye4Software B.V. | Overijssel |
| Eyesiu Medicines B.V. | |
| F-ACT IT | Groningen |
| F.Institute B.V. | |
| Faber Electronics B.V. | Gelderland |
| FabFilter | North Holland |
| Facilitor | Overijssel |
| FacilityApps | North Holland |
| Factris Holding B.V. | |
| Fagron | South Holland |
| FairCode B.V. | |
| Fairphone | North Holland |
| Famas FM Solutions B.V. | North Brabant |
| Fameus Automatisering B.V. | Gelderland |
| Fantazm | North Brabant |
| FareHarbor B.V. | |
| Faster Forward B.V. | North Brabant |
| Fastned | North Holland |
| FastNet Africa B.V. | |
| Fastware B.V. | North Brabant |
| FC-IT | North Holland |