| Twentyseven Global, LLC | Kansas |
| TwentySix2 Marketing, LLC | Georgia |
| TWG, Inc. | Kentucky |
| Twiage Solutions, Inc. | New York |
| Twiangulate | North Carolina |
| Twilight Technology, Inc. | California |
| Twilio, Inc. | California |
| TWIML | Missouri |
| Twin Brook Capital Partners | Illinois |
| Twin Circles eMedia | Minnesota |
| Twin Cities Linux Users Group | Minnesota |
| Twin Cities PC User Group | Minnesota |
| Twin Cities PHP Users Group | Minnesota |
| Twin Cities Spine Center | |
| TWIN Communications | Texas |
| Twin County Tech, Inc. | Virginia |
| Twin Data Corporation | New Jersey |
| Twin Industries | California |
| Twin Oaks Computing, Inc. | |
| Twin Rivers Communications | Florida |
| Twin State Technical Services Ltd | Iowa |
| Twin Sun Solutions | |
| Twin Valley | |
| Twine | California |
| Twine Data | California |
| Twine Therapeutics, Inc. | |
| Twingate | California |
| TwinOaks Technology, Inc. | |
| TwinPeaks Online | California |
| Twinstate | New York |
| TwinStrand Biosciences, Inc. | Washington |
| Twinword, Inc. | California |
| TwinzAPP | Florida |
| Twio | Utah |
| Twist | California |
| Twist Bioscience | California |
| Twisted Melon, Inc. | New York |
| Twisted Pair Networking | Minnesota |
| Twisted Pixel Games, LLC | Texas |
| Twistlab | Utah |
| TWiT | California |
| Twitag | Texas |
| Twitch Interactive, Inc. | California |
| Twitchy Thumbs Entertainment, Inc. | California |
| Twitter, Inc. | California |
| TwitterGadget | Illinois |
| Twixtext | Florida |
| TWN Communications | Florida |
| Two Barrels, LLC | |
| Two Bit Solutions | |