| Two Black Nerds | |
| Two Boxes, Inc. | |
| Two Bulls | New York |
| Two Crows Consulting | Maryland |
| Two Goats | |
| Two Plus A | |
| Two River Technology Group, LLC | New Jersey |
| Two Rivers Marketing | |
| Two Sigma Investments, L.P. | New York |
| Two Technologies, Inc. | Pennsylvania |
| Two Techs Computer Support | Missouri |
| Two Wheels Marketing | |
| Twocanoes Software, Inc. | |
| TwoCans Technologies, Inc. | |
| TwoConnect | Florida |
| Twofold Media, LLC | |
| TwoLabs | |
| Twomile Heavy Industries, Inc. | |
| TwoSense.ai | New York |
| TwoStep Therapeutics | |
| TWST Events | |
| TWV Capital Management, LLC | |
| TWYST Creative, Inc. | |
| Twyxter, Inc. | |
| Tx Systems, Inc. | California |
| TXI, Inc. | Illinois |
| TxnLab, Inc. | |
| TXRX Labs | Texas |
| TXT: Teens Exploring Technology | |
| TXTImpact | New York |
| Txtwire Technologies | Idaho |
| Tyan Computer | California |
| Tyba Energy | |
| Tybang, Inc. | Washington |
| Tyber Medical, LLC | Pennsylvania |
| TYBR Health, Inc. | |
| Tychon Bioscience | Minnesota |
| Tycon Systems, Inc. | Utah |
| Tyfone, Inc. | |
| Tygart Technology, Inc. | West Virginia |
| Tyler Analytics Corporation | Illinois |
| Tyler Junior College | Texas |
| Tyler Sticka | Oregon |
| Tyler Technologies | Texas |
| TylerNet, Inc. | Florida |
| TYLT Ventures | California |
| Tymefly, Inc. | |
| Tymely, Inc. | |
| Tymora Analytical Operations | Indiana |
| Tympanogen | Virginia |