| Laserserve4less | California |
| LaserSoftware, Inc. | Maryland |
| LaserSubstrates, Inc. | Florida |
| LaserZone 1, Inc. | California |
| LASO Health, LLC | |
| Lassen Therapeutics | |
| Lasso AI, Inc. | |
| Lassogen, Inc. | |
| Lassospace | Texas |
| Last 7 Studios | Florida |
| Last Energy, Inc. | |
| Last Lock, Inc. | Wisconsin |
| Lastline, Inc. | California |
| LastMile AI, Inc. | |
| LastPass | Massachusetts |
| Latakoo | |
| Latch Mobile, LLC | |
| LatchBio | California |
| Latchel | Washington |
| Late Night Internet Marketing | Texas |
| Latent AI, Inc. | New Jersey |
| LatentSpace Therapeutics, LLC | |
| Latham Biopharm Group | Maryland |
| Lathem Time Corporation | Georgia |
| Latigo Biotherapeutics | |
| Latigo Optics, Inc. | California |
| Latin Made | |
| Latin Marketing | |
| Latinas in Tech | |
| Latinbits, LLC | Florida |
| Latino Graphics | |
| Latitude | Massachusetts |
| Latitude 41 Capital | Florida |
| Latitude Consulting, Inc. | Utah |
| Latitude Media, Inc. | |
| Latitude Pharmaceuticals, Inc. | |
| Lattery Creations, LLC | Georgia |
| Lattice | California |
| Lattice Automation, Inc. | |
| Lattice Semiconductor | Oregon |
| Lattice Technology, Inc. | Colorado |
| LatticeWork, Inc. | |
| Lattix, Inc. | Massachusetts |
| Latus Bio, Inc. | |
| Lauder Partners, LLC | |
| Laughing Buddha Games | California |
| Laughing Penguin Productions | New York |
| Laughing Rock Technology | |
| Laughing Squid Hosting | California |
| Laughingbird Software, LLC | California |